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Figure 5


Fig. 5. Forespore membrane growth is defective in the slk1{Delta} mutant. (A) Time-lapse experiments showing forespore membrane growth in wild-type (S1478) and slk1{Delta} (S1883) strains carrying the plasmid pREP81-Psy1-GFP. Cells were sporulated on MEA plates and DNA was stained with Hoechst (blue). Stacks of eight images separated by 0.5 µm were taken every 2 minutes. Deconvolution of images and maximal projections were obtained using Deltavision software. GFP and Hoechst merged images were generated with ImageJ. Scale bars: 4 µm. (B) Forespore membrane (FSM) growth stops prematurely in slk1{Delta} cells. The FSM length of wild-type and slk1{Delta} spores shown in A was measured, and means and standard deviations are represented.