Fig. 5. Myosin II and Myosin VI act at distinctive steps in the same pathway to localize Miranda. Live imaging of embryos co-expressing Miranda-GFP (green) and Histone2A-mRFP (red) injected with buffer as a control (A), a Rho kinase inhibitor to downregulate Myosin II (B), treated by MyoVI RNAi (C), or both RKI and MyoVI RNAi (D). After injections, embryos were fixed and stained with anti-Miranda antibody (green). DNA, red.