Fig. 2. Formation and clearance of the single large FAG produced by the continuous presence of Jpk in the culture medium. (A-L) Vero cells (control, A) were treated with low concentrations of Jpk (50 nM), and at different times were fixed and stained with TRITC-phalloidin. Examples of F-actin punctae (B-D,G-K, arrowheads), F-actin amorphous aggregates (B-E,G-I, arrows) and the FAG (F) are shown. Inset in D is an enlargement of the circled area, to better visualize F-actin punctae and F-actin amorphous aggregate structures. Scale bar: 10 µm.