Fig. 9. Cells can generate segregated aggresomes with different molecular compositions. Vero cells were transfected with a GFP-huntingtin mutant plasmid (GFP-htt^m). (A) At early expression times of GFP-htt^m (4 hours), a diffuse cytoplasmic staining was seen (green). (B,C) Subsequent treatment with Jpk (+Jpk, 50 nM) produced F-actin amorphous aggregates and a FAG (red) as long as the GFP-htt^m aggresome was also forming (green). (D,E) After longer Jpk treatment, the FAG is fragmented in F-actin amorphous aggregates and F-actin punctae (D, red) until its complete clearance (E), but the GFP-htt^m aggresome (E, green) remained in the cytoplasm. (F) Notice that the two aggresomes, one containing GFP-htt^m (green) and the other F-actin (red), constantly remain morphologically and molecularly segregated, even when cells were submitted to various Jpk pulses (also see supplementary material Fig. S1). Scale bar: 10 µm.