Fig. 7. Depletion of ABBA alters lamellipodial dynamics. (A) Time-lapse movies of C6-R cells transfected with control or ABBA siRNA oligonucleotides were analysed by drawing a one-pixel-wide line across lamellipodium in the direction of protrusion. The kymograph was constructed by copying the image from this line from 200 frames of the movie and pasting along the x-axis. Steeper slopes in the kymograph correspond to higher velocity rates in the lamellipodial protrusion. (B) Velocity of individual protrusions was calculated and plotted in the graph. Data were collected from 98 ABBA-knockdown and 105 control protrusion events, error bars represent s.e.m. (C) Individual ruffling events (>0.5 µm) were calculated from kymographs of 20 cells. Data are represented as mean ± s.e.m. ^**P<0.02; ^*P<0.05; Student's t-test. Scale bar: 5 µm.