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Figure 2


Fig. 2. Skeletal growth is reduced in Col2a1-ICAT transgenic mice. (A) Structure of the transgene construct and the priming sites for genotyping the Col2a1-ICAT transgenic mice. (B) PCR results; genotyping of Col2a1-ICAT transgenic mice. Mice 1, 2, 4 and 5 (lanes 1, 2, 4 and 5) are positive for Flag-ICAT transgene expression (P, positive control; N, negative control). (C) Body weight of 2-week-old and 4-week-old transgenic mice and WT littermates. A significant reduction in body weight was observed in Col2a1-ICAT transgenic mice. (D) Radiographic analysis showing the delay in the longitudinal growth of 1-week-old, 2-week-old and 4-week-old Col2a1-ICAT transgenic mice compared with their WT littermates. (E,F) Alizarin Red and Alcian Blue staining showed that skeletal development was relatively normal in 1-day-old new born Col2a1-ICAT transgenic mice. By contrast, a significant reduction in postnatal skeletal growth was observed in 2-week-old Col2a1-ICAT transgenic mice.