Fig. 3. The N-terminus and PDZ domain of DLG-1 are sufficient for membrane association in dlg-1(ok318) and dlg-1(ok318);ajm-1(RNAi) embryos. (A) Diagram displaying DLG-1::GFP deletion constructs and their localization in wild-type and dlg-1(ok318) embryos. (B,F,J) All constructs localized properly along the junctions of epidermal cells in wild-type embryos. (C,G,K) Localization of DLG::GFP deletions in dlg-1(ok318) homozygous mutants. (C) DLG-1(1-186)::GFP mislocalized into puncta along the junctional belt, with large regions of the junction lacking GFP. (G) DLG-1(1-468)::GFP localized along the lateral junction, although some gaps were present at the 1.5-fold stage of elongation. (K) DLG-1(1-710)::GFP localization was indistinguishable from wild type in dlg-1(ok318) embryos. (D,H,L) All constructs localized along the junctions of epidermal cells in ajm-1(RNAi) embryos. (E,I,M) Localization of DLG-1::GFP deletions in dlg-1(ok318);ajm-1(RNAi) double-mutant embryos. (E) DLG-1(1-186) was mislocalized away from the junction in the cytoplasm of epidermal cells in double mutants. (I) Localization of DLG-1(1-468)::GFP in dlg-1(ok318);ajm-1(RNAi) double mutants was similar to its localization in dlg-1(ok318) single-mutant embryos (G). (M) DLG-1(1-710)::GFP localization was indistinguishable from wild-type in dlg-1(ok318);ajm-1(RNAi) double mutants. Scale bar: 10 µm.