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Figure 4


Fig. 4. The SH3 domain acts via a LET-413-dependent pathway to mediate lateral distribution of DLG-1 along the junction. (A) DLG-1(1-186)::GFP localization remains punctate throughout elongation in dlg-1(ok318) mutant embryos, demonstrating the importance of the PDZ domains for junctional localization of DLG-1. (B) DLG-1(1-468)::GFP localization is punctate at the beginning of elongation, but its localization becomes more continuous along the junction as elongation proceeds, but cannot restore proper rates of lateral distribution. (C) DLG-1(1-710) GFP is localized evenly along the junction prior to the beginning of elongation. (D) DLG-1({Delta}17-116)::GFP shows normal localization in a dlg-1(ok318) mutant. (E) DLG-1(1-710)::GFP in let-413 mutants displays delayed rates of lateral distribution, similar to DLG-1(1-468) in dlg-1(ok318) embryos (B). (F) DLG-1(1-468)::GFP shows similar rates of lateral distribution in dlg-1(ok318);let-413(RNAi) double mutants compared to dlg-1(ok318) alone (B). Scale bar: 10 µm.