Fig. 6. Glycine but not serines are required for activity of the CT45-59 axonal signal. (A) The A/D PI of NgCAM (n=23), LCT (n=24), LexNct45-59 (n=38) and its mutants LexNct45-59(G-R) (n=41) and LexNct45-59(S-A) (n=30) (as indicated on left) were determined. Error bars correspond to s.e.m. ^***P<0.0001. (B,C) The localization of LexNct45-59(G-R) (B) and LexNct45-59(S-A) (C) is shown. Surface distribution of the chimeras was detected with an anti-Lex antibody without permeabilization (green). DsRed was co-expressed (red) to delineate all neuronal processes. The soma regions containing the dendrites are shown in the top row, whereas the distal regions of the long axons are shown in the bottom row for both B and C.