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Figure 3


Fig. 3. TbG63 is a binding partner for TbRab1A. Soluble, recombinant His-TbG63 (His-TbG{Delta}TM) was incubated with the GTP-locked (Q-Rab-GST) and GDP-locked (S-Rab-GST) mutants of TbRab1A (lanes 1 and 2), GST alone (lane 3), or wild type TbRab1A (wt-Rab-GST) (lanes 4 and 5). The Rab proteins were preloaded with either GTP{gamma}S (lanes 1, 4) or GDP (lanes 2, 5). Bound proteins were eluted with GDP-EDTA and fractionated by SDS-PAGE followed by immuno-blotting for TbG63 (upper panel) or GST (lower panel).