Fig. 5. Overexpression of TbG63 affects Golgi morphology. (A) Control cells (first row) or those expressing full-length TbG63 tagged with either YFP or BB2 were fixed and labeled for Golgi (GRASP) or TGN (GRIP), and DNA (DAPI). (B) Cells expressing an N-terminally truncated TbG63 (YFP-TbG63N) were fixed and labeled for Golgi (GRASP) and DNA (DAPI). Note that the absence of the N-terminus prevents changes to Golgi morphology. Scale bar: 1 µm. (C) Cryo-iEM of parasites overexpressing full-length YFP-TbG63 visualized using antibodies to GFP followed by 10 nm protein-A-gold. Control, untransfected cell. The three different phenotypes of altered Golgi morphology are arciform, vesiculated and tubulo-vesiculated. Scale bar: 0.25 µm.