Fig. 8. VSG export in bloodstream parasites is not affected by depletion of TbG63. Pulse-chase kinetics analysis of VSG arrival at the cell surface. (A) Autoradiograph of pulse-labeled material recovered from soluble (S) and pellet (P) fractions following purification with ConA-Sepharose, from control, non-induced parasites (ND) or parasites induced with doxycyclin for 72 hours to deplete TbG63 (DOX). (B) Western blot of cells used for the VSG export assay, probed with anti-TbG63 antibody to demonstrate depletion of the protein and anti-tubulin as loading control. (C) Quantification of data obtained from A.