Fig. 2. Mug27 is a meiosis-specific SPB-associated protein. (A,B) Mug27 subcellular localization during meiosis relative to Sid4-mRFP (A) and Sad1-mCherry (B) expression as observed by fluorescence microscopy. The mug27⁺-gfp sid4⁺-mrfp strain (SOP028; A) and the mug27⁺-gfp sad1⁺-mCherry strain (SOP062; B) were induced to enter meiosis by nitrogen starvation. 10 hours later, the cells were collected and stained with Hoechst 33342 to visualize the DNA (blue). The GFP signal is green, and the mRFP and mCherry signals are red. (B; lowermost panels) Enlarged views of the merged images at early anaphase II (left) and late anaphase II (right) at the areas indicated by the rectangles are shown. (C) Mug27-GFP does not localize to the SPB in mitotic cells. Mitotic cells were transformed with empty pREP1-GFP (GFP expression vector; SOP065) or pREP1-mug27⁺-GFP (Mug27-GFP expression vector; SOP066), which overproduce (OP) the encoded protein. The sid2⁺-gfp sad1⁺-mCherry strain (SOP067) served as an SPB colocalization control.