Fig. 3. Accurate positioning of Mug27 at anaphase II depends on proper FSM formation. (A) The homothallic haploid strain SOP069 (h⁹⁰ mug27⁺-gfp sad1⁺-mCherry spo15) was cultured in EMM2 with appropriate supplements and then transferred to EMM-N to induce meiosis. 10 hours later, cells at different stages of meiosis were stained with Hoechst 33342. The bar graphs were drawn by determining the Mug27-GFP localization in 69 spo15⁺ and 87 spo15 cells, and then plotting the frequencies of the indicated patterns. (B) The haploid strains SOP044 (h^– mug27⁺-gfp pat1-114; i) and SOP079 (h^– mug27⁺-gfp cdc11-123 pat1-114; ii) were monitored after shifting the temperature to induce meiosis. 5 hours later, cells at different stages of meiosis were stained with Hoechst 33342 (blue). Scale bars: 5 µm. (C) The bar graphs were drawn by determining the Mug27-GFP localization in 114 pat1-114 and 112 cdc11-123 pat1-114 cells, and then plotting the frequencies of the indicated patterns.