Fig. 6. Vacuole morphology during sporulation. GFP-Ypt7-expressing mug27⁺ (SOP122w) and mug27 (SOP123) cells were cultured in EMM2, then induced to enter meiosis by nitrogen starvation at 25°C. 10 hours later, cells at different stages of meiosis were stained with FM4-64 and Hoechst 33342. The formation of the FSM in mug27⁺ (i) and mug27 (ii) cells during spore formation was visualized using fluorescence microscopy to monitor the behavior of FM4-64 and the vacuole membranes. Scale bar: 10 µm. (iii) Cells in which vacuole-membrane fusion was observed (yellow) or not (green) at late sporulation in mug27⁺ (n=161) or mug27 (n=162) cells, respectively. Typical images are shown in the upper panel.