JCS -- Armstrong et al. 122 (1): 136 Figure IG2

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. BzATP-induced translocation of PKC ${alpha}$ is transient. (A) An osteoclast-like cell expressing PKC ${alpha}$ -EGFP was stimulated with BzATP (150 µM) at 60 seconds, causing transient redistribution of the fluorescence from the cytosol to the periphery of the cell. (B) Plot represents the time course of the membrane localization, with the time of addition of BzATP indicated in this and subsequent figures by the vertical broken line. Translocation of PKC ${alpha}$ to the membrane was transient, with recovery by 600 seconds. Some photobleaching of EGFP occurred at longer recording times. Duration at half peak of responses was 258±26 seconds (mean ± s.e.m., n=16 cells).