JCS -- Armstrong et al. 122 (1): 136 Figure IG4

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. BzATP fails to cause translocation of PKC ${alpha}$ in osteoclasts derived from the bone marrow of P2rx7^–/– mice. (A) PKC ${alpha}$ -EGFP was located in the cytosol of osteoclasts generated from wild-type (WT) mice, and addition of vehicle alone had no effect. Addition of BzATP (150 µM), however, did cause the translocation of PKC to the membrane in wild-type osteoclasts. (B) Addition of BzATP (150 µM) to osteoclasts that were generated from P2rx7^–/– mice failed to elicit translocation of PKC ${alpha}$ . However, addition of PMA (10 µM) did induce translocation to the membrane. Images were obtained from cells that were fixed 1-2 minutes after addition of the agonist. Red horizontal lines indicate the location of the reconstructed z-stacks shown below. Blue horizontal lines indicate the location of the x-y confocal images shown above. Data are representative of three independent experiments (Table 1).