Fig. 7. BzATP-induced translocation of PKC is dependent on extracellular Ca²⁺. Osteoclast-like cells were bathed in medium containing EGTA (4 mM) to chelate extracellular Ca²⁺. (A) BzATP (150 µM) was applied at 60 seconds, but no translocation was observed (representative of responses in 14 cells). Subsequently, PMA (10 µM) was added to the bath at 830 seconds as a positive control, and did induce translocation of PKC-EGFP to the membrane. (B) Membrane localization plotted for cell shown in A reveals no effect of BzATP in the absence of Ca²⁺ (BzATP added to bath at the time indicated by the initial broken line). However, translocation was induced by PMA added at the time indicated by the second broken line. Data are representative of more than five independent experiments.