JCS -- Compagnon et al. 122 (1): 25 Figure IG5

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Fig. 5. Rab5 controls the removal of PtdIns(4,5)P₂ from early endocytic intermediate membrane. (A-D) PH(PLC ${delta}$ )-GFP localization in wt (A) and rab5²-GLCs (B-D) stage 9 egg chambers. (C) Inset on the anterior margin. (D) Cross-section through the broken line in B. Note the presence of large PLC(PLC ${delta}$ )-GFP-positive structures in the oocyte cytoplasm when Rab5 is absent which are never seen in control (A) (projection of optical sections). Scale bars: 50 µm, except in C, 25 µm. (E-H) YP1 and PH(PLC ${delta}$ )-GFP localization in wt (E) and rab5² GLC (F-H) stage 10 egg chambers. (G; red in E,H) YP1; (F; green in E,H) PH(PLC ${delta}$ )-GFP; (blue in E,H) DNA. (Inset, E) In controls, YP1 is found throughout the cytoplasm. (H) When Rab5 is absent, YP1 discrete staining inside the oocyte cytoplasm is lost and it is found on large PH(PLC ${delta}$ )-GFP-positive structures in the oocyte cytoplasm. Scale bars: 50 µm. (I-L) ${alpha}$ -adaptin and PH(PLC ${delta}$ )-GFP localization in wt (I) and rab5² GLC (J-L) stage 9 egg chambers. (K; red in I,L) ${alpha}$ -adaptin; (J; green in I,L) PH(PLC ${delta}$ )-GFP. Note that, when Rab5 is absent, large PH(PLC ${delta}$ )-GFP-positive structures in the oocyte cytoplasm are associated with ${alpha}$ -adaptin. Scale bars: 30 µm. (M-T) F-actin and PH(PLC ${delta}$ )-GFP localization in wt (M,Q) and rab5² GLC (N-P,R-T) stage 9 egg chambers. (O,S; red in M,P,Q,T) F-actin; (N,R; green in M,P,Q,T) PH(PLC ${delta}$ )-GFP. Note that the large PH(PLC ${delta}$ )-GFP-positive structures in the oocyte cytoplasm observed when Rab5 is absent contain F-actin (projection of optical sections).