Fig. 8. The Inv protein is a dynamic component along the primary cilium. (A) Schematic representation of FRAP analysis in Inv-GFP cells. Formvar film on which ciliated cells were cultured was folded over onto itself with the cell side out. Inv-GFP was partially photobleached. (B) Representative data of side-view FRAP. Photobleaching was performed at time 0. Bleaching widths of each FRAP experiment were 0.31 µm (top), 0.52 µm (middle) and 0.26 µm (bottom). The bleaching point is indicated by a yellow arrowhead. Line scans of fluorescent signal from `*' to `**' are shown below. Inv-GFP fluorescence rapidly recovered after photobleaching in all experiments, and the bleaching gap almost completely recovered within 60 seconds. Scale bar: 5 µm.