Fig. 4. Exocytosis of BDNF-EGFP-containing DCVs in neuronal soma and neurites. Exocytosis of BDNF-EGFP in the soma (A) and neurites (B) was imaged using methods similar to those used in Fig. 3. Exocytosis of BDNF-EGFP-containing DCVs was not accompanied by a fluorescence cloud or by a significant loss of fluorescence. Curves shown are averages of ten individual events with standard deviations indicated. Shown below the curves are images of representative DCVs at 1.0-second time intervals. Start points of the exocytic events, occurring at various times following depolarization, are indicated by arrows.