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Figure 8


Fig. 8. Expression of constitutively active MEK1 or p38 MAPK induces GATA4 nuclear translocation and cellular maturation in integrin-β1-null endoderm cells. (A,B) Integrin-β1-null endoderm cells were transfected with HA-tagged constitutively active (CA) MEK1 or control vector pcDNA3. The transfected cells were grown on fibronectin for 2 days and stained for HA-tag, GATA4 and Dab2. In untransfected cells, GATA4 was found mainly in the cytoplasm (red arrows). HA–CA-MEK1 expression led to GATA4 nuclear localization and increased Dab2 expression (white arrowheads). Some of the transfected cells spread on fibronectin and formed FAs containing HA–CA-MEK1 (white arrows). (C) Western blots show increased expression of laminin, nidogen and Dab2 in HA–CA-MEK1-transfected cells. (D) Integrin-β1-null endoderm cells were transfected with CA HA-tagged MKK6-p38{alpha} or the non-phosphorylatable mutant MKK6-p38{alpha}/AGF. The transfected cells were stained for HA-tag, GATA4 and Dab2. (E) Western blots show increased expression of laminin, nidogen and Dab2 in MKK6-p38{alpha}-transfected cells.