Fig. 10. BAPTA-AM treatment alters the uropod length of JAM-A^+/+ neutrophils. Actin immunofluorescence staining of JAM-A^–/– and ^+/+ murine neutrophils is shown. To block integrin internalization, cells were pre-incubated with BAPTA-AM where indicated. The number of cells showing long tails was increased upon BAPTA-AM treatment of JAM-A^+/+ cells, whereas uropod lengths were not altered in JAM-A^–/– neutrophils. Cell nuclei are counterstained with DAPI (blue). Scale bar: 5 µm. The length of neutrophil tails was measured using Carnoy software version 2.0 for Macintosh and the frequency of cells with different tail size is reported for the two populations (JAM-A^+/+, black columns; JAM-A^–/–, white columns). The graph shows the means ± s.e.m. of four experiments. ^*P<0.01, Student's t-test.