Fig. 5. JAM-A colocalizes with 5β1 and β2 integrins. Double staining of JAM-A (green) and 5β1 integrins (red) or JAM-A (red) and β2 integrin (green) in polarized dHL60 cells. The cells were seeded on fibronectin-coated coverslips, treated with fMLP for 20 minutes and incubated with anti-JAM-A and anti-β1 integrin antibodies before paraformaldehyde fixation. Each panel represents a single projection of a z-stack of about three confocal section (stack z-spacing, 0.4 µm). Merged images show JAM-A colocalization with integrins. Cell nuclei are counterstained with DAPI (blue). Scale bar: 5 µm. The figure shows one representative experiment out of five performed.