Fig. 7. Integrins induce JAM-A clustering. Immunofluorescence staining of dHL60 cells incubated with beads coated with JAM-A mAb (BV16) or a β1 integrin pAb, mouse or rabbit IgGs respectively. Cells were fixed and stained as in Fig. 6. β1-integrin-pAb-coated beads induced JAM-A clustering (arrows), whereas JAM-A-mAb-coated beads were unable to recruit integrin β1. Beads coated with either mouse or rabbit IgGs were not effective. Single projections of z-stacks of about two confocal sections (stack z-spacing, 0.3 µm) are shown. Scale bars: 5 µm. The figure shows one representative experiment out of two performed.