Fig. 8. JAM-A concentrates in intracellular vesicles in murine neutrophils. Immunofluorescence analysis of neutrophils derived from bone marrow of JAM-A^+/+ and JAM-A^–/– mice. Living cells were treated with an anti-murine JAM-A mAb (BV12) and then seeded on fibronectin-coated coverslips and fixed. Untreated (a,b) or WKYMVm-activated (c,d) cells are shown. The nuclei of the cells are counterstained with DAPI (blue). Murine neutrophils show JAM-A localization at intracellular vesicles, whereas, as expected, JAM-A was undetectable in JAM-A^–/– cells (b,d). Scale bars: 5 µm. The figure shows one representative experiment out of four performed.