Fig. 5. The integrin 6β1 mediates cell spreading on endogenous Ln-332 when 3β1 is absent. MK3⁺ and MK3^– cells were seeded on Ln-332 (A) or Col-1 (B), allowed to spread, and then incubated with 6-blocking antibody GoH3 (10 µg/ml). After 3 hours, the number of spread cells was scored and expressed as a percentage of the total number of cells. In each independent experiment, approximately 500 cells were counted for each condition. The graphs depict the averages of three experiments (^*P<0.05, ^***P<0.0005). Scale bar: 50 µm.