Fig. 6. Ln-332 synthesis and deposition are not affected by the deletion of the integrin 3 subunit. (A) MK3⁺ and MK3^– cells were seeded on Col-I and then detached with EDTA at the indicated time points after which the ECM was dissolved in sample buffer, subjected to SDS-PAGE, and the 2 chain of Ln-332 was detected by western blotting. (B) Immunofluorescence images demonstrating patches of deposited Ln-332 in spread MK3⁺ cells (left panel) and MK3^– cells (right panel). Scale bar: 10 µm. (C) Low-magnification immunofluorescence images demonstrating Ln-332 trails left behind by MK3⁺ cells (left), and MK3^– cells (right) migrating over Col-I. Scale bar: 10 µm.