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Figure 2


Fig. 2. Binding of laminin-1 and recombinant LG4 protein to GM1. (A,B) Binding of (A) laminin-1 (1 ng/well) and (B) recombinant LG4 protein (1 ng/well) to glycosphingolipids (5 µg/well) was measured by ELISA. Data are given as the means + standard deviations (+ s.d.) of experiments performed in triplicate. (C) Dose-dependent inhibitory effects of various amounts of the recombinant LG4 protein on the binding of laminin-1 (1 ng/well) to GM1 (5 µg/well)-coated plates. Bound laminin-1 was immunostained using antibody aganst laminin-{alpha}1–LG1-3, which recognizes laminin-1 but not LG4, and then quantified. (D) Dose-dependent inhibitory effects of laminin-1 on binding of biotin-bound AG73 to GM1-coated plates (5 µg/well). Each value is given as the mean (+ s.d.) of three experiments.