Fig. 5. Changes in emerin and lamins A/C expression upon downregulation of β-catenin with siRNA. (A) PCR with primers specific for β-actin and β-catenin. (B,E) Emerin and lamins A/C expression by RT-PCR against β-actin. (C,F) Immunoblotting of whole-cell extracts or nuclei with emerin and lamins A/C antibody, together with β-actin loading control. (D,G) Cells were stained with emerin or lamins A/C antibodies. Bars: 10 µm.