Fig. 7. USP36 is required for a normal nucleolar structure. (A-D'') HeLa cells transfected with the USP36 siRNA-1 (A-A'', B-B'', and D-D'') or mock (C-C'') vector and stained with anti-USP36 antibody (A-D) with anti-nucleolus (A',B') or anti-NPM (C',D') antibody. A''-D'' are merged images. Asterisks in A-A'' and B-B'' indicate USP36-depleted cells. Scale bars: 10 µm. (E,F) EM of HeLa cells transfected with mock vector (E) or USP36 siRNA-1 vector (F). N and arrowheads indicate the nucleoplasm and nucleoli, respectively. Insets show high-magnification images of the nucleoli. In nucleoli, the granular components (asterisks), and the dense fibrillar components (arrows) surrounding the fibrillar center, are indicated. Scale bars: 5 µm.