Fig. 3. Dynamics of neuromast deposition. (A-E') Shown is the process of neuromast deposition within a wild-type embryo that was labelled with the vital membrane marker BODIPY ceramide and followed over a 10-minute period by time-lapse analysis. Rosettes are indicated by brackets and the plane of tissue separation is indicated by arrowheads. (A'-E') Details of cellular cell shape changes along the plane of tissue separation indicate that cells surrounding individual rosettes are extensively elongated and that tissue separation occurs exclusively in between interneuromast cells, leaving individual rosettes intact. Therefore, rosettes, including their surrounding cells, are units of tissue separation.