Fig. 2. Effect of BI1 on the expression of P450 2E1. (A) Neo and BI1 cells were immunoblotted with anti-P450 2E1 and β-actin antibodies. (B) Neo and BI1 cells were treated with 5 µM thapsigargin or 5 µg/ml tunicamycin for 0, 2, 4, 6, 8 and 12 hours. Cell lysates were immunoblotted with anti-P450 2E1 and β-actin antibodies. The expression of P450 2E1 was normalized to that of β-actin as a relative ratio. The data indicate the mean±s.e.m. (n=7). ^*P<0.05, significantly different from the amount of P450 2E1 expressed in the Neo cells without any treatment; ^#P<0.05, significantly different from the amount of P450 2E1 expressed in the Neo cells under each of the indicated conditions.