Fig. 3. KIF3b colocalizes with p0071. (A) MCF-7 cells were co-stained for KIF3b and p0071 or KIF3b and -tubulin, respectively. In the upper panel a monoclonal KIF3b antibody (clone 35, BD Transduction Laboratories) was used and in the lower panel KIF3b was stained using a polyclonal antibody (H60, Santa Cruz). In interphase, KIF3b (green) is localized at the centrosome and colocalizes with p0071 (red, upper panel) and -tubulin (red, lower panel). Both KIF3b antibodies (green) stain primarily the centrosomal region. (B) HeLa cells were synchronized and analyzed during cytokinesis. For staining of KIF3b the polyclonal antibody was used. P0071 (green) accumulated at the contractile ring whereas KIF3b (red) localized at the microtubule midzone, which is closely associated with the contractile ring. Scale bars: 10 µm, enlargements 5 µm.