Fig. 6. CTGF is necessary for enhanced adhesion, invasion and proliferation of HT1080 cells stably transfected with LC3-WT. (A) Western blot of CTGF protein (top) and qRT-PCR of Ctgf mRNA (bottom) in vector- and LC3-WT-transfected HT1080 cells transiently transfected with control siRNA or CTGF. Both CTGF protein and mRNA were efficiently knocked down following fibronectin siRNA transfection for 72 hours, in both vector- and LC3-WT-transfected HT1080 cells. (B-D) Adhesion (B), invasion (C) and proliferation (D) of HT1080 cells stably transfected with LC3-WT (as judged by MTT assay) compared in transfected cells transiently cotransfected with control siRNA or CTGF siRNA. Cell adhesion was assessed for cells cultured with or without fibronectin coating of the dishes (B). Bars denote mean ± s.e.m. of three experiments; ^*P<0.05; ^** P<0.01; ^***P<0.005.