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Journal of Cell Science, Vol 10, 27-46, Copyright © 1972 by Company of Biologists

Submitted on June 10, 1971

Dna Synthesis in Purified Populations of Avian Erythroid Cells

A. F. WILLIAMS 1

1 Department of Biochemistry, University of Adelaide, Australia; Department of Biochemistry, University of Oxford, England

By the use of equilibrium density-gradient centrifugation erythroblasts and early polychromatic erythrocytes have been isolated from avian anaemic bone marrow. Cells from both the unfractionated and purified preparations have been characterized in terms of their histological type, size, haemoglobin content and ability to synthesize DNA. Erythroblasts were the only cells to synthesize DNA and it appeared that their progeny, the polychromatic erythrocyte, failed to enter a new S phase. The experimental system described allows biochemical characterization of earlier stages of avian erythropoiesis than has previously been possible.

Submitted on June 10, 1971




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© The Company of Biologists Ltd 1972