spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Billia, F.
Right arrow Articles by de Boni, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Billia, F.
Right arrow Articles by de Boni, U.

Journal of Cell Science, Vol 100, Issue 1 219-226, Copyright © 1991 by Company of Biologists

JOURNAL ARTICLES

Localization of centromeric satellite and telomeric DNA sequences in dorsal root ganglion neurons, in vitro

F Billia and U de Boni
Department of Physiology, Faculty of Medicine, University of Toronto, Ontario, Canada.

Chromatin domains of interphase nuclei are organized in a tissue-specific, non-random manner. In the present work, the spatial arrangement of satellite (sDNA) and telomeric (tDNA) DNA was examined in nuclei of murine Dorsal Root Ganglion (DRG) cells, maintained in vitro. In situ hybridization in conjunction with three-dimensional reconstruction was employed. A mean number of 8.02 +/- 0.40 sDNA signals/nucleus was detected, of which 41.65 +/- 0.59% were associated with the nucleolus. The remaining fraction of signals was localized between the nucleolus and the nuclear membrane. sDNA signals were reproducibly localized at a mean distance of 3.15 +/- 0.06 microns from the nuclear center and measured 1-2 microns in diameter. Given a centromere complement of 40 per murine nucleus, the relatively low number of signals detected and their large signal volumes were interpreted to reflect clustering of centromeres, a phenomenon common in mammalian cells. An average of 37.00 +/- 1.52 tDNA signals was detected per nucleus. Of these, and in contrast to sDNA signals, only 18.45 +/- 0.41% of these signals were associated with the nucleolus while the remainder was distributed between the nucleolus and the nuclear membrane. Both centromeric and telomeric signals often occurred in pairs and were distributed throughout the nucleoplasm. No evidence for a classical Rabl configuration was found.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. Nagele, A. Velasco, W. Anderson, D. McMahon, Z Thomson, J Fazekas, K Wind, and H Lee
Telomere associations in interphase nuclei: possible role in maintenance of interphase chromosome topology
J. Cell Sci., January 1, 2001; 114(2): 377 - 388.
[Abstract] [PDF]

Home page
 J. Cell Biol.Home page
K. Weipoltshammer, C. Schofer, M. Almeder, V. V. Philimonenko, K. Frei, F. Wachtler, and P. Hozak
Intranuclear Anchoring of Repetitive DNA Sequences: Centromeres, Telomeres, and Ribosomal DNA
J. Cell Biol., December 27, 1999; 147(7): 1409 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. Janevski, P. C. Park, and U. De Boni
Changes in Morphology and Spatial Position of Coiled Bodies during NGF-induced Neuronal Differentiation of PC12 Cells
J. Histochem. Cytochem., November 1, 1997; 45(11): 1523 - 1532.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1991