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Journal of Cell Science, Vol 100, Issue 1 243-247, Copyright © 1991 by Company of Biologists

JOURNAL ARTICLES

Lepidopteran cell variants resistant to 5-bromodeoxyuridine and their use for transfection of the HSV-TK gene

WD Xie, DF He, XZ Wang, QX Long and ZL Pu
Research Institute of Entomology, Zhongshan University, Guangzhou, People's Republic of China.

Following mutagenesis of cultured lepidopteran cells (Spodoptera frugiperda) by ethylmethanesulfonate, three variants resistant to 5-bromodeoxyuridine (BrdUrd) were isolated. These clones were 100- to 200-fold more resistant to BrdUrd than the parental cells and were shown to be deficient in thymidine kinase (TK). The drug-resistant phenotype was stable for up to three years of culture under nonselective conditions. It was also found that the S. frugiperda cell line was highly resistant to aminopterin. A selective medium was formulated and used to select herpes simplex virus thymidine kinase (HSV-TK) transfectants from the TK-deficient cells.




© The Company of Biologists Ltd 1991