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Journal of Cell Science, Vol 101, 13-24, Copyright © 1992 by Company of Biologists

Submitted on July 16, 1991
Accepted on October 7, 1991

Parental Genome Separation in Reconstructions of Somatic and Premeiotic Metaphases of Hordeum Vulgare x H. Bulbosum

T. SCHWARZACHER 1, J. S. HESLOP-HARRISON 1, K. ANAMTHAWAT-JÓNSSON 1, R. A. FINCH 2, and M. D. BENNETT 2

1 Karyobiology Group, Department of Cell Biology, JI Centre for Plant Science Research, Norwich NR4 7UJ, UK
2 Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK

Author for correspondence

A stable interspecific sexual plant hybrid between Hordeum vulgare cv. Tuleen 346 (barley) x H. bulbosum was shown to have seven chromosomes originating from each parent by genomic in situ hybridization. Electron microscope serial thin-section reconstructions of metaphases and comparison with light micrograph karyo-types enabled chromosomes to be identified from their morphology. The three-dimensional positions of their centromeres were established and analysed in the reconstructions of somatic root tip metaphases and cells at mitotic metaphase near their entry into meiosis. Parental genomes tended to lie in spatially separated domains in both tissues. Although varying in morphology, the two sets of chromosomes had similar mean sizes, so size differences did not cause the separation observed. In the EM, the centromere-associated structures of the chromosomes of the more central genome, originating from H. vulgare, were larger than those of the more peripheral genome of H. bulbosum origin.

Key words: nuclear organization, nuclear architecture, three-dimensional reconstruction, meiosis, Hordeum, genome separation

Submitted on July 16, 1991
Accepted on October 7, 1991


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© The Company of Biologists Ltd 1992