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Journal of Cell Science, Vol 101, Issue 2 463-474, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
H Epstein, TC James and PB Singh
Department of Genetics, University of Cambridge, UK.
The mealybug chromosome cycle is one of the most dramatic examples of genomic imprinting known. In embryos that are to become male the entire paternal chromosome set becomes heterochromatic and inactive at the blastoderm stage, while the maternal set remains active and euchromatic. HP1 is a protein from Drosophila melanogaster, which binds preferentially to heterochromatin on polytene chromosomes and is likely to be a modifier of position effect variegation. This paper describes the isolation and sequencing of two cDNA clones encoding HP1 homologs from the mealybug, Planococcus citri. The protein product of the cDNA clone that was closer to HP1 in sequence was expressed as a fusion protein in Escherichia coli, and polyclonal rat antibodies were raised against it. Immunohistochemistry to mealybug squash preparations showed that this protein was a male-specific nuclear protein, but that it was not specifically associated with the heterochromatic set of chromosomes.
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S. Bongiorni, M. Mazzuoli, S. Masci, and G. Prantera Facultative heterochromatization in parahaploid male mealybugs: involvement of a heterochromatin-associated protein Development, October 1, 2001; 128(19): 3809 - 3817. [Abstract] [Full Text] [PDF]
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 J. F. Smothers and S. Henikoff The Hinge and Chromo Shadow Domain Impart Distinct Targeting of HP1-Like Proteins Mol. Cell. Biol., April 1, 2001; 21(7): 2555 - 2569. [Abstract] [Full Text]
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G. Wang, A. Ma, C.-m. Chow, D. Horsley, N. R. Brown, I. G. Cowell, and P. B. Singh Conservation of Heterochromatin Protein 1 Function Mol. Cell. Biol., September 15, 2000; 20(18): 6970 - 6983. [Abstract] [Full Text]
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 S. Bongiorni, O. Cintio, and G. Prantera The Relationship Between DNA Methylation and Chromosome Imprinting in the Coccid Planococcus citri Genetics, April 1, 1999; 151(4): 1471 - 1478. [Abstract] [Full Text]
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 S Messmer, A Franke, and R Paro Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster. Genes & Dev., July 1, 1992; 6(7): 1241 - 1254. [Abstract] [PDF]
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