Journal of Cell Science, Vol 101, Issue 3 547-559, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis

M Hatsumi and SA Endow
Department of Microbiology & Immunology, Duke University Medical Center, Durham, North Carolina 27710.

The Drosophila microtubule motor protein, nonclaret disjunctional (ncd), is required for proper chromosome distribution in meiosis and mitosis. We have examined the meiotic and mitotic divisions in wild-type Drosophila oocytes and early embryos, and the effects of three ncd mutants (cand, ncd and ncdD) on spindle structure and chromosome movement. The ncd mutants cause abnormalities in spindle structure early in meiosis I, and abnormal chromosome configurations throughout meiosis I and II. Defective divisions continue in early embryos of the motor null mutant, cand, with abnormal early mitotic spindles. The effects of mutants on spindle structure suggest that ncd is required for proper meiotic spindle assembly, and may play a role in forming or maintaining spindle poles in meiosis. The disruption of normal meiotic and mitotic chromosome distribution by ncd mutants can be attributed to its role as a spindle motor, although a role for ncd as a chromosome-associated motor protein is not excluded. The ncd motor protein functions not only in meiosis, but also performs an active role in the early mitotic divisions of the embryo.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Cai, L. N. Weaver, S. C. Ems-McClung, and C. E. Walczak Kinesin-14 Family Proteins HSET/XCTK2 Control Spindle Length by Cross-Linking and Sliding Microtubules Mol. Biol. Cell, March 1, 2009; 20(5): 1348 - 1359. [Abstract] [Full Text] [PDF]

				C. F. Cullen, A. L. Brittle, T. Ito, and H. Ohkura The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster J. Cell Biol., November 21, 2005; 171(4): 593 - 602. [Abstract] [Full Text] [PDF]

				J. K. Jang, T. Rahman, and K. S. McKim The Kinesinlike Protein Subito Contributes to Central Spindle Assembly and Organization of the Meiotic Spindle in Drosophila Oocytes Mol. Biol. Cell, October 1, 2005; 16(10): 4684 - 4694. [Abstract] [Full Text] [PDF]

				H. N. Skold, D. J. Komma, and S. A. Endow Assembly pathway of the anastral Drosophila oocyte meiosis I spindle J. Cell Sci., April 15, 2005; 118(8): 1745 - 1755. [Abstract] [Full Text] [PDF]

				J. C. Ambrose, W. Li, A. Marcus, H. Ma, and R. Cyr A Minus-End-directed Kinesin with Plus-End Tracking Protein Activity Is Involved in Spindle Morphogenesis Mol. Biol. Cell, April 1, 2005; 16(4): 1584 - 1592. [Abstract] [Full Text] [PDF]

				A. I. Marcus, W. Li, H. Ma, and R. J. Cyr A Kinesin Mutant with an Atypical Bipolar Spindle Undergoes Normal Mitosis Mol. Biol. Cell, April 1, 2003; 14(4): 1717 - 1726. [Abstract] [Full Text] [PDF]

				B. J. Tunquist and J. L. Maller Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs Genes & Dev., March 15, 2003; 17(6): 683 - 710. [Full Text] [PDF]

				K. L. Giunta, J. K. Jang, E. A. Manheim, G. Subramanian, and K. S. McKim subito Encodes a Kinesin-like Protein Required for Meiotic Spindle Pole Formation in Drosophila melanogaster Genetics, April 1, 2002; 160(4): 1489 - 1501. [Abstract] [Full Text] [PDF]

				R. M. Q. Shanks, R. J. Kamieniecki, and D. S. Dawson The Kar3-Interacting Protein Cik1p Plays a Critical Role in Passage Through Meiosis I in Saccharomyces cerevisiae Genetics, November 1, 2001; 159(3): 939 - 951. [Abstract] [Full Text] [PDF]

				M. B. Gordon, L. Howard, and D. A. Compton Chromosome Movement in Mitosis Requires Microtubule Anchorage at Spindle Poles J. Cell Biol., February 5, 2001; 152(3): 425 - 434. [Abstract] [Full Text] [PDF]

				H. Ohkura, M. A. Garcia, and T. Toda Dis1/TOG universal microtubule adaptors - one MAP for all? J. Cell Sci., January 11, 2001; 114(21): 3805 - 3812. [Abstract] [Full Text] [PDF]

				A. E. Brent, A. MacQueen, and T. Hazelrigg The Drosophila wispy Gene Is Required for RNA Localization and Other Microtubule-Based Events of Meiosis and Early Embryogenesis Genetics, April 1, 2000; 154(4): 1649 - 1662. [Abstract] [Full Text]

				M. Riparbelli, G Callaini, and D. Glover Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila J. Cell Sci., January 9, 2000; 113(18): 3341 - 3350. [Abstract] [PDF]

				V. Mountain, C. Simerly, L. Howard, A. Ando, G. Schatten, and D. A. Compton The Kinesin-Related Protein, Hset, Opposes the Activity of Eg5 and Cross-Links Microtubules in the Mammalian Mitotic Spindle J. Cell Biol., October 18, 1999; 147(2): 351 - 366. [Abstract] [Full Text] [PDF]

				T. Megraw, K Li, L. Kao, and T. Kaufman The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila Development, January 7, 1999; 126(13): 2829 - 2839. [Abstract] [PDF]

				S. Bonaccorsi, M. G. Giansanti, and M. Gatti Spindle Self-organization and Cytokinesis During Male Meiosis in asterless Mutants of Drosophila melanogaster J. Cell Biol., August 10, 1998; 142(3): 751 - 761. [Abstract] [Full Text] [PDF]

				S. Endow and D. Komma Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes J. Cell Sci., January 9, 1998; 111(17): 2487 - 2495. [Abstract] [PDF]

				C Gonzalez, G Tavosanis, and C Mollinari Centrosomes and microtubule organisation during Drosophila development J. Cell Sci., January 9, 1998; 111(18): 2697 - 2706. [Abstract] [PDF]

				C. A. Bascom-Slack and D. S. Dawson The Yeast Motor Protein, Kar3p, Is Essential for Meiosis I J. Cell Biol., October 20, 1997; 139(2): 459 - 467. [Abstract] [Full Text] [PDF]

				S. A. Endow and D. J. Komma Spindle Dynamics during Meiosis in Drosophila Oocytes J. Cell Biol., June 16, 1997; 137(6): 1321 - 1336. [Abstract] [Full Text] [PDF]

				C. E. Walczak, S. Verma, and T. J. Mitchison XCTK2: A Kinesin-related Protein That Promotes Mitotic Spindle Assembly in Xenopus laevis Egg Extracts J. Cell Biol., February 24, 1997; 136(4): 859 - 870. [Abstract] [Full Text] [PDF]

				B. Williams, A. Dernburg, J Puro, S Nokkala, and M. Goldberg The Drosophila kinesin-like protein KLP3A is required for proper behavior of male and female pronuclei at fertilization Development, January 6, 1997; 124(12): 2365 - 2376. [Abstract] [PDF]

				D. Komma and S. Endow Enhancement of the ncdD microtubule motor mutant by mutants of alpha Tub67C J. Cell Sci., January 1, 1997; 110(2): 229 - 237. [Abstract] [PDF]

				S. Endow and D. Komma Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins J. Cell Sci., January 10, 1996; 109(10): 2429 - 2442. [Abstract] [PDF]

				M. Riparbelli and G Callaini Meiotic spindle organization in fertilized Drosophila oocyte: presence of centrosomal components in the meiotic apparatus J. Cell Sci., January 5, 1996; 109(5): 911 - 918. [Abstract] [PDF]

				K. S. McKim and R. S. Hawley Chromosomal Control of Meiotic Cell Division Science, December 8, 1995; 270(5242): 1595 - 1601. [Abstract] [PDF]

				S. Endow, R Chandra, D. Komma, A. Yamamoto, and E. Salmon Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis J. Cell Sci., January 4, 1994; 107(4): 859 - 867. [Abstract] [PDF]

				H White-Cooper, L Alphey, and D. Glover The cdc25 homologue twine is required for only some aspects of the entry into meiosis in Drosophila J. Cell Sci., January 12, 1993; 106(4): 1035 - 1044. [Abstract] [PDF]

				R Chandra, S. Endow, and E. Salmon An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays J. Cell Sci., January 3, 1993; 104(3): 899 - 906. [Abstract] [PDF]