Journal of Cell Science, Vol 102, Issue 4 729-737, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Relocation of nucleolar proteins around chromosomes at mitosis. A study by confocal laser scanning microscopy

T Gautier, M Robert-Nicoud, MN Guilly and D Hernandez-Verdun
Institut Jacques Monod, Paris, France.

The behaviour of nucleolar antigens known to associate with chromosomes at mitosis was investigated in mammalian cells (HeLa, HEp-2, PtK1, CHO) by immunofluorescence and confocal laser scanning microscopy. Serial optical sections through mitotic cells, from prophase to telophase, were used to generate three-dimensional images of the antigen distribution. Our results indicate that, at the onset of mitosis, these antigens leave the nucleoli in a highly ordered manner to form a network extending from the nucleoli towards the nuclear envelope. The migration begins at very early prophase, when the condensation of the chromosomes is not yet visible. After completion of the migration at late prophase, the labelling is found at the chromosome periphery. The antigens remain distributed as a sheath surrounding the chromosomes from prophase to telophase. Therefore, the proteins involved in the formation of this perichromosomal layer have different behaviour than those of the prenucleolar bodies. The antigens appear to interact strongly with chromosomes, since they are not lost during chromosome isolation in hypotonic buffer. Each chromosome is entirely covered from one telomere to the other, except in the centromeric region. Thus the relocation of these nucleolar proteins does not appear to be the result of a passive accumulation at the chromosome periphery, but seems rather to be due to an active targeting to specific sites. Consequently, these proteins may have a determining function in the progression of the cells through mitosis, possibly by participating in the protection and stabilization of the chromosomes.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				V. K. Nayyar, K. Shire, and L. Frappier Mitotic chromosome interactions of Epstein-Barr nuclear antigen 1 (EBNA1) and human EBNA1-binding protein 2 (EBP2) J. Cell Sci., December 1, 2009; 122(23): 4341 - 4350. [Abstract] [Full Text] [PDF]

				S. Hussain, S. B. Benavente, E. Nascimento, I. Dragoni, A. Kurowski, A. Gillich, P. Humphreys, and M. Frye The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability J. Cell Biol., July 13, 2009; 186(1): 27 - 40. [Abstract] [Full Text] [PDF]

				N. Angelier, M. Tramier, E. Louvet, M. Coppey-Moisan, T. M. Savino, J. R. De Mey, and D. Hernandez-Verdun Tracking the Interactions of rRNA Processing Proteins during Nucleolar Assembly in Living Cells Mol. Biol. Cell, June 1, 2005; 16(6): 2862 - 2871. [Abstract] [Full Text] [PDF]

				B. C. Valdez, D. Henning, R. B. So, J. Dixon, and M. J. Dixon The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factor PNAS, July 20, 2004; 101(29): 10709 - 10714. [Abstract] [Full Text] [PDF]

				D. Hernandez-Verdun, P. Roussel, and J. Gebrane-Younes Emerging concepts of nucleolar assembly J. Cell Sci., January 6, 2002; 115(11): 2265 - 2270. [Abstract] [Full Text] [PDF]

				T. Dousset, C. Wang, C. Verheggen, D. Chen, D. Hernandez-Verdun, and S. Huang Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription Mol. Biol. Cell, August 1, 2000; 11(8): 2705 - 2717. [Abstract] [Full Text]

				M. BIGGIOGERA and C. PELLICCIARI Heterogeneous ectopic RNP-derived structures (HERDS) are markers of transcriptional arrest FASEB J, April 1, 2000; 14(5): 828 - 834. [Abstract] [Full Text]

				V. Sirri, P. Roussel, and D. Hernandez-Verdun In Vivo Release of Mitotic Silencing of Ribosomal Gene Transcription Does Not Give Rise to Precursor Ribosomal RNA Processing J. Cell Biol., January 24, 2000; 148(2): 259 - 270. [Abstract] [Full Text] [PDF]

				T. Savino, R Bastos, E Jansen, and D Hernandez-Verdun The nucleolar antigen Nop52, the human homologue of the yeast ribosomal RNA processing RRP1, is recruited at late stages of nucleologenesis J. Cell Sci., January 6, 1999; 112(12): 1889 - 1900. [Abstract] [PDF]

				J. M. Westendorf, K. N. Konstantinov, S. Wormsley, M.-D. Shu, N. Matsumoto-Taniura, F. Pirollet, F. G. Klier, L. Gerace, and S. J. Baserga M Phase Phosphoprotein 10 Is a Human U3 Small Nucleolar Ribonucleoprotein Component Mol. Biol. Cell, February 1, 1998; 9(2): 437 - 449. [Abstract] [Full Text]

				N Fomproix, J Gebrane-Younes, and D Hernandez-Verdun Effects of anti-fibrillarin antibodies on building of functional nucleoli at the end of mitosis J. Cell Sci., January 2, 1998; 111(3): 359 - 372. [Abstract] [PDF]

				A. Beven, R Lee, M Razaz, D. Leader, J. Brown, and P. Shaw The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs J. Cell Sci., January 6, 1996; 109(6): 1241 - 1251. [Abstract] [PDF]

				I. Kill Localisation of the Ki-67 antigen within the nucleolus. Evidence for a fibrillarin-deficient region of the dense fibrillar component J. Cell Sci., January 6, 1996; 109(6): 1253 - 1263. [Abstract] [PDF]

				M Starborg, K Gell, E Brundell, and C Hoog The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression J. Cell Sci., January 1, 1996; 109(1): 143 - 153. [Abstract] [PDF]

				N Gilbert, L Lucas, C Klein, M Menager, N Bonnet, and D Ploton Three-dimensional co-location of RNA polymerase I and DNA during interphase and mitosis by confocal microscopy J. Cell Sci., January 1, 1995; 108(1): 115 - 125. [Abstract] [PDF]

				M. Azum-Gelade, J Noaillac-Depeyre, M Caizergues-Ferrer, and N Gas Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase J. Cell Sci., January 2, 1994; 107(2): 463 - 475. [Abstract] [PDF]