|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 1 201-209, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
JA Swanson, A Locke, P Ansel and PJ Hollenbeck
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.
In murine bone marrow-derived macrophages, lysosomes often form tubulovesicular compartments, whose extended distribution in the cytoplasm depends on the integrity of cytoplasmic microtubules. When macrophages with fluorescently labeled lysosomes were plated onto coverslips opsonized with IgG, they engaged that surface in a phagocytic response (frustrated phagocytosis). The tubular lysosomal compartment of these cells collected in a central, perinuclear region, despite the continued presence of a radiating array of cytoplasmic microtubules. Using methods developed in the study of melanophores, we permeabilized macrophages engaged in frustrated phagocytosis, then re-activated lysosome extension along microtubules. Permeabilization was selective for plasma membranes, in that high molecular weight probes such as trypan blue or IgG could enter cells, while fluorescent probes previously loaded into lysosomes via endocytosis remained contained therein. Addition of 2 mM ATP, GTP or UTP to these permeabilized cell models produced centrifugal extension of tubular lysosomes. Selective depletion of ATP, using Escherichia coli glycerol kinase, inhibited ATP-dependent extension but not that which occurred with GTP or UTP, indicating that the mechanism of radial movement can use any of these three nucleotide triphosphates. Extension was independent of pH between 6.8 and 7.4, and was inhibited by AMP-PNP and by GMP-PNP. Depolymerization of cytoplasmic microtubules with nocodazole prevented subsequent ATP-inducible lysosome extension, whereas preincubation of cells with cytochalasin D did not inhibit the response. These results are consistent with the in vitro mechanochemical properties of kinesin (Cohn et al., 1989), and support earlier evidence, obtained in living cells, that kinesin is the mechanochemical motor of lysosome extension along microtubules in macrophages.
This article has been cited by other articles:
|
|
 |
|
  N. Nabavi, Y. Urukova, M. Cardelli, J. E. Aubin, and R. E. Harrison Lysosome Dispersion in Osteoblasts Accommodates Enhanced Collagen Production during Differentiation J. Biol. Chem., July 11, 2008; 283(28): 19678 - 19690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dzakpasu and D. Axelrod Dynamic Light Scattering Microscopy. A Novel Optical Technique to Image Submicroscopic Motions. II: Experimental Applications Biophys. J., August 1, 2004; 87(2): 1288 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Opal, J. J. Garcia, F. Propst, A. Matilla, H. T. Orr, and H. Y. Zoghbi Mapmodulin/Leucine-rich Acidic Nuclear Protein Binds the Light Chain of Microtubule-associated Protein 1B and Modulates Neuritogenesis J. Biol. Chem., September 5, 2003; 278(36): 34691 - 34699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. de Figueiredo, A. Doody, R. S. Polizotto, D. Drecktrah, S. Wood, M. Banta, M. S. Strang, and W. J. Brown Inhibition of Transferrin Recycling and Endosome Tubulation by Phospholipase A2 Antagonists J. Biol. Chem., December 7, 2001; 276(50): 47361 - 47370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Guerin and C. de Chastellier Pathogenic Mycobacteria Disrupt the Macrophage Actin Filament Network Infect. Immun., May 1, 2000; 68(5): 2655 - 2662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Wubbolts, M Fernandez-Borja, I Jordens, E Reits, S Dusseljee, C Echeverri, R. Vallee, and J Neefjes Opposing motor activities of dynein and kinesin determine retention and transport of MHC class II-containing compartments J. Cell Sci., January 3, 1999; 112(6): 785 - 795. [Abstract] [PDF]
|
|
|
|
 |
|
 R. S. Labow, E. Meek, and J. P. Santerre Synthesis of Cholesterol Esterase by Monocyte-Derived Macrophages: A Potential Role in the Biodegradation of Poly(Urethane)s J Biomater Appl, January 1, 1999; 13(3): 187 - 205. [Abstract] [PDF]
|
|
|
|
 |
|
 D. M. Ward, J. D. Leslie, and J. Kaplan Homotypic Lysosome Fusion in Macrophages: Analysis Using an In Vitro Assay J. Cell Biol., November 3, 1997; 139(3): 665 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Huber, R Saffrich, M Anton, M Passreiter, W Ansorge, K Gorgas, and W Just A heterotrimeric G protein-phospholipase A2 signaling cascade is involved in the regulation of peroxisomal motility in CHO cells J. Cell Sci., January 12, 1997; 110(23): 2955 - 2968. [Abstract] [PDF]
|
|
|
|
|
|
A. Bakker, P Webster, W. Jacob, and N. Andrews Homotypic fusion between aggregated lysosomes triggered by elevated [Ca2+]i in fibroblasts J. Cell Sci., January 9, 1997; 110(18): 2227 - 2238. [Abstract] [PDF]
|
|
|
|
|
|
F. Rajas, V. Gire, and B. Rousset Involvement of a Membrane-bound Form of Glutamate Dehydrogenase in the Association of Lysosomes to Microtubules J. Biol. Chem., November 22, 1996; 271(47): 29882 - 29890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Overly, H. Rieff, and P. Hollenbeck Organelle motility and metabolism in axons vs dendrites of cultured hippocampal neurons J. Cell Sci., January 5, 1996; 109(5): 971 - 980. [Abstract] [PDF]
|
|
|
|
|
|
L. K. Opresko, C.-P. Chang, B. H. Will, P. M. Burke, G. N. Gill, and H. S. Wiley Endocytosis and Lysosomal Targeting of Epidermal Growth Factor Receptors Are Mediated by Distinct Sequences Independent of the Tyrosine Kinase Domain J. Biol. Chem., March 3, 1995; 270(9): 4325 - 4333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Perou and J Kaplan Chediak-Higashi syndrome is not due to a defect in microtubule-based lysosomal mobility J. Cell Sci., January 9, 1993; 106(1): 99 - 107. [Abstract] [PDF]
|
|
