Journal of Cell Science, Vol 103, Issue 2 309-320, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles

JH Henson, D Nesbitt, BD Wright and JM Scholey
Department of Biology, Dickinson College, Carlisle, PA 17013.

We have recently used domain-specific monoclonal antibodies (mAbs) to immunofluorescently localize kinesin to vesicle-like structures in the cytoplasm of sea urchin coelomocytes. In order to characterize further these localization patterns we have examined the distribution of kinesin with respect to the arrangement of microtubules (MTs) and various organelles. In double-label experiments involving the immunofluorescent staining of kinesin (using a mixture of the mAbs SUK2, 4 and 5), MTs were labeled with an antiserum against sea urchin tubulin, the endoplasmic reticulum (ER) was labeled with an antiserum against a luminal calsequestrin-like protein, the Golgi apparatus was labeled with rhodamine-wheat germ agglutinin (WGA) or NBD-ceramide, mitochondria were labeled with rhodamine 123, endosomes were labeled with Texas Red-ovalbumin, and lysosomes were labeled with Lucifer yellow or acridine orange. Kinesin-labeled vesicle-like structures were found in the same regions of the cells as MTs and the ER, being widely distributed in motile cells, but restricted to the perinuclear regions of stationary cells. There also appeared to be a correlation between the distribution of endosomes and kinesin staining in a subpopulation of cells. The kinesin binding structures were found occasionally to align in linear arrays, consistent with the idea that kinesin may transport ER and endosomes along linear MT tracks. No clear correlations were observed between the kinesin staining and the distribution of mitochondria, the Golgi apparatus or lysosomes, suggesting that kinesin may specifically associate with only a subclass of organelles in coelomocytes.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				V. Brockton, J. H. Henson, D. A. Raftos, A. J. Majeske, Y.-O. Kim, and L. C. Smith Localization and diversity of 185/333 proteins from the purple sea urchin - unexpected protein-size range and protein expression in a new coelomocyte type J. Cell Sci., February 1, 2008; 121(3): 339 - 348. [Abstract] [Full Text] [PDF]

				Y. Du, S. Ferro-Novick, and P. Novick Dynamics and inheritance of the endoplasmic reticulum J. Cell Sci., June 15, 2004; 117(14): 2871 - 2878. [Abstract] [Full Text] [PDF]

				J. H. Henson, R. Nazarian, K. L. Schulberg, V. A. Trabosh, S. E. Kolnik, A. R. Burns, and K. J. McPartland Wound Closure in the Lamellipodia of Single Cells: Mediation by Actin Polymerization in the Absence of an Actomyosin Purse String Mol. Biol. Cell, March 1, 2002; 13(3): 1001 - 1014. [Abstract] [Full Text] [PDF]

				J. H. Henson, T. M. Svitkina, A. R. Burns, H. E. Hughes, K. J. MacPartland, R. Nazarian, and G. G. Borisy Two Components of Actin-based Retrograde Flow in Sea Urchin Coelomocytes Mol. Biol. Cell, December 1, 1999; 10(12): 4075 - 4090. [Abstract] [Full Text]

				J. D. Lane and V. J. Allan Microtubule-based Endoplasmic Reticulum Motility in Xenopus laevis: Activation of Membrane-associated Kinesin during Development Mol. Biol. Cell, June 1, 1999; 10(6): 1909 - 1922. [Abstract] [Full Text]

				A. Khodjakov, E. M. Lizunova, A. A. Minin, M. P. Koonce, and F. K. Gyoeva A Specific Light Chain of Kinesin Associates with Mitochondria in Cultured Cells Mol. Biol. Cell, February 1, 1998; 9(2): 333 - 343. [Abstract] [Full Text]

				H. V. Goodson, S. J. Kang, and S. A. Endow Molecular phylogeny of the kinesin family of microtubule motor proteins J. Cell Sci., July 1, 1994; 107(7): 1875 - 1884. [Abstract] [PDF]

				V. Allan and R. Vale Movement of membrane tubules along microtubules in vitro: evidence for specialised sites of motor attachment J. Cell Sci., July 1, 1994; 107(7): 1885 - 1897. [Abstract] [PDF]

				D. Marks, J. Larkin, and M. McNiven Association of kinesin with the Golgi apparatus in rat hepatocytes J. Cell Sci., January 9, 1994; 107(9): 2417 - 2426. [Abstract] [PDF]

				L D'Andrea, M. Danon, G. Sgourdas, and E. Bonder Identification of coelomocyte unconventional myosin and its association with in vivo particle/vesicle motility J. Cell Sci., January 8, 1994; 107(8): 2081 - 2094. [Abstract] [PDF]

				S. Lin and C. Collins Regulation of the intracellular distribution of cytoplasmic dynein by serum factors and calcium J. Cell Sci., January 6, 1993; 105(2): 579 - 588. [Abstract] [PDF]