Journal of Cell Science, Vol 103, Issue 2 397-405, Copyright © 1992 by Company of Biologists

JOURNAL ARTICLES

Inhibition by kinase inhibitors of the development and the disappearance of the preprophase band of microtubules in tobacco BY-2 cells

J Katsuta and H Shibaoka

The effects of two kinase inhibitors, staurosporine and K-252a, on the development and the disappearance of the preprophase band of microtubules (PPB) were studied in tobacco BY-2 cells, the cell cycle of which had been synchronized. Treatment of cells at late G2 phase or at prophase with 20 micromolar staurosporine or 2 micromolar K- 252a for 2 or 3 h caused a decrease in the rate of development of PPBs and inhibited the disappearance of PPBs once they had developed, with the resultant accumulation of cells with a PPB. Cortical microtubules (MTs) or PPBs on protoplast ghosts, which were prepared from BY-2 cells in late G2 phase or at prophase, disappeared on treatment of the ghosts with ATP or ATP(gamma)S but not on treatment with AMP-PNP. The effect of ATP on the disappearance of MTs on the ghosts was suppressed by 20 micromolar staurosporine. Although 2 micromolar K-252a applied to the culture medium suppressed the anticipated disappearance of PPBs, it did not suppress the disappearance of MTs on the ghosts that was caused by ATP. We propose tentatively that a kinase sensitive to staurosporine but not to K-252a is directly involved in the disappearance of MTs and that a kinase that is sensitive to K-252a is indirectly involved in this process. The disappearance of MTs on protoplast ghost that was induced by ATP did not occur in the presence of 20 micromolar taxol.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. Forer The diversity of cytoplasmic microtubules J. Cell Sci., January 1, 2008; 121(1): 7 - 9. [Full Text] [PDF]

				P. Dhonukshe and T. W. J. Gadella Jr. Alteration of Microtubule Dynamic Instability during Preprophase Band Formation Revealed by Yellow Fluorescent Protein-CLIP170 Microtubule Plus-End Labeling PLANT CELL, March 1, 2003; 15(3): 597 - 611. [Abstract] [Full Text] [PDF]

				M. Weingartner, P. Binarova, D. Drykova, A. Schweighofer, J.-P. David, E. Heberle-Bors, J. Doonan, and L. Bogre Dynamic Recruitment of Cdc2 to Specific Microtubule Structures during Mitosis PLANT CELL, August 1, 2001; 13(8): 1929 - 1943. [Abstract] [Full Text] [PDF]

				C Granger and R Cyr Use of abnormal preprophase bands to decipher division plane determination J. Cell Sci., January 2, 2001; 114(3): 599 - 607. [Abstract] [PDF]

				L. Bögre, O. Calderini, P. Binarova, M. Mattauch, S. Till, S. Kiegerl, C. Jonak, C. Pollaschek, P. Barker, N. S. Huskisson, et al. A MAP Kinase Is Activated Late in Plant Mitosis and Becomes Localized to the Plane of Cell Division PLANT CELL, January 1, 1999; 11(1): 101 - 114. [Abstract] [Full Text]

				D. A. Collings, T. Asada, N. S. Allen, and H. Shibaoka Plasma Membrane-Associated Actin in Bright Yellow 2 Tobacco Cells . Evidence for Interaction with Microtubules Plant Physiology, November 1, 1998; 118(3): 917 - 928. [Abstract] [Full Text]

				C. Camilleri, J. Azimzadeh, M. Pastuglia, C. Bellini, O. Grandjean, and D. Bouchez The Arabidopsis TONNEAU2 Gene Encodes a Putative Novel Protein Phosphatase 2A Regulatory Subunit Essential for the Control of the Cortical Cytoskeleton PLANT CELL, April 1, 2002; 14(4): 833 - 845. [Abstract] [Full Text] [PDF]