|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 3 653-664, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
O Kagami and R Kamiya
Dynein was extracted from outer arm-less axonemes of the mutant oda1 and fractionated by high-pressure liquid chromatography on a MonoQ column into seven distinct subspecies (named a-g). Each subspecies contained one or two heavy chains and several medium-sized and light chains; by vanadate/UV-induced photocleavage and SDS- polyacrylamide gel electrophoresis, eight distinct heavy chains were identified. Analysis of the mutant axonemes indicated that the subspecies f (containing two heavy chains) is missing in the inner-arm mutant ida1 and the subspecies a, c and d are missing in the mutant ida4. Six subspecies (all but f) supported microtubule translocation with the maximal rate ranging from 2 to 12 micrometre s-1 and the apparent Km for ATP ranging from about 10 to 100 micromolar. All the subspecies translocated microtubules with the plus end leading, indicating that all the inner-arm dyneins are minus end-directed motors. Five subspecies (all but b and f) displayed microtubule rotation during translocation at rates of up to about 10 Hz. Unexpectedly, the Km values for ATP for translocation and rotation did not always agree; because of this, the pitch of the movement was variable with some subspecies. These observations indicate that axonemes are equipped with several inner-arm subspecies and that torque generation is a feature common to many of them.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. H. Bui, H. Sakakibara, T. Movassagh, K. Oiwa, and T. Ishikawa Asymmetry of inner dynein arms and inter-doublet links in Chlamydomonas flagella J. Cell Biol., August 10, 2009; 186(3): 437 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wirschell, C. Yang, P. Yang, L. Fox, H.-a. Yanagisawa, R. Kamiya, G. B. Witman, M. E. Porter, and W. S. Sale IC97 Is a Novel Intermediate Chain of I1 Dynein That Interacts with Tubulin and Regulates Interdoublet Sliding Mol. Biol. Cell, July 1, 2009; 20(13): 3044 - 3054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bower, K. VanderWaal, E. O'Toole, L. Fox, C. Perrone, J. Mueller, M. Wirschell, R. Kamiya, W. S. Sale, and M. E. Porter IC138 Defines a Subdomain at the Base of the I1 Dynein That Regulates Microtubule Sliding and Flagellar Motility Mol. Biol. Cell, July 1, 2009; 20(13): 3055 - 3063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yagi, K. Uematsu, Z. Liu, and R. Kamiya Identification of dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella J. Cell Sci., May 1, 2009; 122(9): 1306 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Furuta, T. Yagi, H.-a. Yanagisawa, H. Higuchi, and R. Kamiya Systematic Comparison of in Vitro Motile Properties between Chlamydomonas Wild-type and Mutant Outer Arm Dyneins Each Lacking One of the Three Heavy Chains J. Biol. Chem., February 27, 2009; 284(9): 5927 - 5935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Bui, H. Sakakibara, T. Movassagh, K. Oiwa, and T. Ishikawa Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella J. Cell Biol., December 1, 2008; 183(5): 923 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Liu, H. Takazaki, Y. Nakazawa, M. Sakato, T. Yagi, T. Yasunaga, S. M. King, and R. Kamiya Partially Functional Outer-Arm Dynein in a Novel Chlamydomonas Mutant Expressing a Truncated {gamma} Heavy Chain Eukaryot. Cell, July 1, 2008; 7(7): 1136 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yamamoto, H.-a. Yanagisawa, T. Yagi, and R. Kamiya Novel 44-Kilodalton Subunit of Axonemal Dynein Conserved from Chlamydomonas to Mammals Eukaryot. Cell, January 1, 2008; 7(1): 154 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hook and R. B. Vallee The dynein family at a glance J. Cell Sci., November 1, 2006; 119(21): 4369 - 4371. [Full Text] [PDF]
|
|
|
|
|
|
T. Yagi, I. Minoura, A. Fujiwara, R. Saito, T. Yasunaga, M. Hirono, and R. Kamiya An Axonemal Dynein Particularly Important for Flagellar Movement at High Viscosity: IMPLICATIONS FROM A NEW CHLAMYDOMONAS MUTANT DEFICIENT IN THE DYNEIN HEAVY CHAIN GENE DHC9 J. Biol. Chem., December 16, 2005; 280(50): 41412 - 41420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Hendrickson, C. A. Perrone, P. Griffin, K. Wuichet, J. Mueller, P. Yang, M. E. Porter, and W. S. Sale IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending Mol. Biol. Cell, December 1, 2004; 15(12): 5431 - 5442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Ersfeld and K Gull Targeting of cytoskeletal proteins to the flagellum of Trypanosoma brucei J. Cell Sci., January 1, 2001; 114(1): 141 - 148. [Abstract] [PDF]
|
|
|
|
|
|
M. E. Porter and W. S. Sale The 9 + 2 Axoneme Anchors Multiple Inner Arm Dyneins and a Network of Kinases and Phosphatases That Control Motility J. Cell Biol., November 27, 2000; 151(5): 37 - 42. [Full Text] [PDF]
|
|
|
|
|
|
C. A. Perrone, S. H. Myster, R. Bower, E. T. O'Toole, and M. E. Porter Insights into the Structural Organization of the I1 Inner Arm Dynein from a Domain Analysis of the 1beta Dynein Heavy Chain Mol. Biol. Cell, July 1, 2000; 11(7): 2297 - 2313. [Abstract] [Full Text]
|
|
|
|
|
|
S. King AAA domains and organization of the dynein motor unit J. Cell Sci., January 7, 2000; 113(14): 2521 - 2526. [Abstract] [PDF]
|
|
|
|
|
|
H Bannai, M Yoshimura, K Takahashi, and C Shingyoji Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella J. Cell Sci., January 3, 2000; 113(5): 831 - 839. [Abstract] [PDF]
|
|
|
|
|
|
S. H. Myster, J. A. Knott, K. M. Wysocki, E. O'Toole, and M. E. Porter Domains in the 1{alpha} Dynein Heavy Chain Required for Inner Arm Assembly and Flagellar Motility in Chlamydomonas J. Cell Biol., August 23, 1999; 146(4): 801 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Porter, R. Bower, J. A. Knott, P. Byrd, and W. Dentler Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas Mol. Biol. Cell, March 1, 1999; 10(3): 693 - 712. [Abstract] [Full Text]
|
|
|
|
|
|
P. Yang and W. S. Sale The Mr 140,000 Intermediate Chain of Chlamydomonas Flagellar Inner Arm Dynein Is a WD-Repeat Protein Implicated in Dynein Arm Anchoring Mol. Biol. Cell, December 1, 1998; 9(12): 3335 - 3349. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Perrone, P. Yang, E. O'Toole, W. S. Sale, and M. E. Porter The Chlamydomonas IDA7 Locus Encodes a 140-kDa Dynein Intermediate Chain Required to Assemble the I1 Inner Arm Complex Mol. Biol. Cell, December 1, 1998; 9(12): 3351 - 3365. [Abstract] [Full Text]
|
|
|
|
|
|
A. Harrison, P. Olds-Clarke, and S. M. King Identification of the t Complex-encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive J. Cell Biol., March 9, 1998; 140(5): 1137 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Sakakibara and H Nakayama Translocation of microtubules caused by the alphabeta, beta and gamma outer arm dynein subparticles of Chlamydomonas J. Cell Sci., January 5, 1998; 111(9): 1155 - 1164. [Abstract] [PDF]
|
|
|
|
|
|
T. Kato-Minoura, M. Hirono, and R. Kamiya Chlamydomonas Inner-Arm Dynein Mutant, ida5, Has a Mutation in an Actin-encoding Gene J. Cell Biol., May 5, 1997; 137(3): 649 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. King and S. K. Dutcher Phosphoregulation of an Inner Dynein Arm Complex in Chlamydomonas reinhardtii Is Altered in Phototactic Mutant Strains J. Cell Biol., January 13, 1997; 136(1): 177 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Habermacher and W. S. Sale Regulation of Flagellar Dynein by Phosphorylation of a 138-kD Inner Arm Dynein Intermediate Chain J. Cell Biol., January 13, 1997; 136(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Tanaka, Z Zhang, and N Hirokawa Identification and molecular evolution of new dynein-like protein sequences in rat brain J. Cell Sci., January 5, 1995; 108(5): 1883 - 1893. [Abstract] [PDF]
|
|
|
|
|
|
D. Asai, S. Beckwith, K. Kandl, H. Keating, H Tjandra, and J. Forney The dynein genes of Paramecium tetraurelia. Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms J. Cell Sci., January 4, 1994; 107(4): 839 - 847. [Abstract] [PDF]
|
|
|
|
|
|
C. Wilkerson, S. King, and G. Witman Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer-arm dynein J. Cell Sci., January 3, 1994; 107(3): 497 - 506. [Abstract] [PDF]
|
|
