|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 3 755-763, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
P Kaur and WG Carter
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Normal human foreskin keratinocytes (HFKs) and transformed HFKs (FEPE1L-8 cells) generated by the introduction of cloned human papillomavirus type 16 sequences were compared for the expression and function of a family of adhesion receptors termed integrins. Initially, cells were examined in conventional monolayer cultures. FEPE1L-8s expressed integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, alpha 6 beta 4 and beta 1 at comparable levels to HFKs. Further, these receptors were fully functional in mediating specific interactions with exogenously supplied ligands. However, FEPE1L-8s exhibited decreased synthesis of a number of extracellular matrix components, including laminin, fibronectin and epiligrin, compared to normal HFKs, which may be an alternate mechanism for regulating adhesion. Subsequently, organotypic cultures (OCs), which provide a suitable in vitro model system for the ordered stratification and differentiation of keratinocytes, were used to study the regulation of integrins and various epidermal markers in normal and transformed cells. OCs consisted of keratinocytes plated on a collagen gel containing primary human fibroblasts, grown at an air-medium interface. Unlike normal HFKs, the transformed FEPE1L-8 cells exhibited (a) disorganized stratification and limited differentiation capacity, (b) invasion into the collagen gel, and (c) unregulated expression of alpha 3 beta 1 and alpha 2 beta 1, and under-expression of alpha 6 beta 4 integrins. Ordered stratification and spatial regulation of integrin expression could be induced in the FEPE1L-8s by substituting Swiss 3T3 fibroblasts in the collagen gel. Further data indicate that the human fibroblasts induce the transformed HFKs to invade into the collagen gel.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Kintarak, S. A. Whawell, P. M. Speight, S. Packer, and S. P. Nair Internalization of Staphylococcus aureus by Human Keratinocytes Infect. Immun., October 1, 2004; 72(10): 5668 - 5675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Maas-Szabowski, A. Starker, and N. E. Fusenig Epidermal tissue regeneration and stromal interaction in HaCaT cells is initiated by TGF-{alpha} J. Cell Sci., July 15, 2003; 116(14): 2937 - 2948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Schmeichel and M. J. Bissell Modeling tissue-specific signaling and organ function in three dimensions J. Cell Sci., June 15, 2003; 116(12): 2377 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Lampe, B. P. Nguyen, S. Gil, M. Usui, J. Olerud, Y. Takada, and W. G. Carter Cellular Interaction of Integrin {alpha}3{beta}1 with Laminin 5 Promotes Gap Junctional Communication J. Cell Biol., December 14, 1998; 143(6): 1735 - 1747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Symington and W. Carter Modulation of epidermal differentiation by epiligrin and integrin alpha 3 beta 1 J. Cell Sci., January 2, 1995; 108(2): 831 - 838. [Abstract] [PDF]
|
|
|
|
|
|
T. Tuan, L. Keller, D Sun, M. Nimni, and D Cheung Dermal fibroblasts activate keratinocyte outgrowth on collagen gels J. Cell Sci., January 8, 1994; 107(8): 2285 - 2289. [Abstract] [PDF]
|
|
