|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 4 1215-1222, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
JR Sims, S Karp and DE Ingber
Department of Surgery, Children's Hospital, Boston, MA.
Studies were carried out with capillary endothelial cells cultured on fibronectin (FN)-coated dishes in order to analyze the mechanism of cell and nuclear shape control by extracellular matrix (ECM). To examine the role of the cytoskeleton in shape determination independent of changes in transmembrane osmotic pressure, membranes of adherent cells were permeabilized with saponin (25 micrograms/ml) using a buffer that maintains the functional integrity of contractile microfilaments. Real-time videomicroscopic studies revealed that addition of 250 microM ATP resulted in time-dependent retraction and rounding of permeabilized cells and nuclei in a manner similar to that observed in intact living cells following detachment using trypsin-EDTA. Computerized image analysis confirmed that permeabilized cells remained essentially rigid in the absence of ATP and that retraction was stimulated in a dose-dependent manner as the concentration of ATP was raised from 10 to 250 microM. Maximal rounding occurred by 30 min with projected cell and nuclear areas being reduced by 69 and 41%, respectively. ATP-induced rounding was also accompanied by a redistribution of microfilaments resulting in formation of a dense net of F-actin surrounding retracted nuclei. Importantly, ATP-stimulated changes in cell, cytoskeletal, and nuclear form were prevented in permeabilized cells using a synthetic myosin peptide (IRICRKG) that has been previously shown to inhibit actomyosin filament sliding in muscle. In contrast, both the rate and extent of cell and nuclear rounding were increased in permeabilized cells exposed to ATP when the soluble FN peptide, GRGDSP, was used to dislodge immobilized FN from cell surface integrin receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Sozo, M. J. Wallace, V. A. Zahra, C. E. Filby, and S. B. Hooper Gene expression profiling during increased fetal lung expansion identifies genes likely to regulate development of the distal airways Physiol Genomics, January 12, 2006; 24(2): 105 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Simpson-Holley, R. C. Colgrove, G. Nalepa, J. W. Harper, and D. M. Knipe Identification and Functional Evaluation of Cellular and Viral Factors Involved in the Alteration of Nuclear Architecture during Herpes Simplex Virus 1 Infection J. Virol., October 15, 2005; 79(20): 12840 - 12851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-H. Lee, C.-L. Chien, H.-K. Liao, Y.-J. Chen, and Z.-F. Chang Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation J. Cell Sci., November 1, 2004; 117(23): 5579 - 5589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Juszczak Comparative Vibrational Spectroscopy of Intracellular Tau and Extracellular Collagen I Reveals Parallels of Gelation and Fibrillar Structure J. Biol. Chem., February 27, 2004; 279(9): 7395 - 7404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Ingber Tensegrity I. Cell structure and hierarchical systems biology J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J Flecknoe, M. J Wallace, R. Harding, and S. B Hooper Determination of alveolar epithelial cell phenotypes in fetal sheep: evidence for the involvement of basal lung expansion J. Physiol., July 1, 2002; 542(1): 245 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Rotundo, T. M. Curtis, M. D. Shah, B. Gao, A. Mastrangelo, S. E. LaFlamme, and T. M. Saba TNF-alpha disruption of lung endothelial integrity: reduced integrin mediated adhesion to fibronectin Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L316 - L329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kostka, R. Giltay, W. Bloch, K. Addicks, R. Timpl, R. Fassler, and M.-L. Chu Perinatal Lethality and Endothelial Cell Abnormalities in Several Vessel Compartments of Fibulin-1-Deficient Mice Mol. Cell. Biol., October 15, 2001; 21(20): 7025 - 7034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Krawczyk and J. M. Penninger Molecular motors involved in T cell receptor clusterings J. Leukoc. Biol., March 1, 2001; 69(3): 317 - 330. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. E. Ingber, S. R. Heidemann, P. Lamoureux, and R. E. Buxbaum Opposing views on tensegrity as a structural framework for understanding cell mechanics J Appl Physiol, October 1, 2000; 89(4): 1663 - 1678. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Langelier, R. Suetterlin, C. D. Hoemann, U. Aebi, and M. D. Buschmann The Chondrocyte Cytoskeleton in Mature Articular Cartilage: Structure and Distribution of Actin, Tubulin, and Vimentin Filaments J. Histochem. Cytochem., October 1, 2000; 48(10): 1307 - 1320. [Abstract] [Full Text]
|
|
|
|
|
|
S. Flecknoe, R. Harding, G. Maritz, and S. B. Hooper Increased lung expansion alters the proportions of type I and type II alveolar epithelial cells in fetal sheep Am J Physiol Lung Cell Mol Physiol, June 1, 2000; 278(6): L1180 - L1185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Helfman, E. T. Levy, C. Berthier, M. Shtutman, D. Riveline, I. Grosheva, A. Lachish-Zalait, M. Elbaum, and A. D. Bershadsky Caldesmon Inhibits Nonmuscle Cell Contractility and Interferes with the Formation of Focal Adhesions Mol. Biol. Cell, October 1, 1999; 10(10): 3097 - 3112. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Cai, L. Pestic-Dragovich, M. E. O'Donnell, N. Wang, D. Ingber, E. Elson, and P. De Lanerolle Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylation Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1349 - C1356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Curtis, R. F. Rotundo, P. A. Vincent, P. J. McKeown-Longo, and T. M. Saba TNF-alpha -induced matrix Fn disruption and decreased endothelial integrity are independent of Fn proteolysis Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L126 - L138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Cipolla, G. Osol, and H. A. Kontos Vascular Smooth Muscle Actin Cytoskeleton in Cerebral Artery Forced Dilatation • Editorial Comment Stroke, June 1, 1998; 29(6): 1223 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pourati, A. Maniotis, D. Spiegel, J. L. Schaffer, J. P. Butler, J. J. Fredberg, D. E. Ingber, D. Stamenovic, and N. Wang Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1283 - C1289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Becker, W. Buchanan, and J. T. Sylvester Protective effects of intravascular pressure and nitric oxide in ischemic lung injury J Appl Physiol, March 1, 1998; 84(3): 803 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Putnam, J. Cunningham, R. Dennis, J. Linderman, and D. Mooney Microtubule assembly is regulated by externally applied strain in cultured smooth muscle cells J. Cell Sci., January 11, 1998; 111(22): 3379 - 3387. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Grabski, E. Arnoys, B. Busch, and M. Schindler Regulation of Actin Tension in Plant Cells by Kinases and Phosphatases Plant Physiology, January 1, 1998; 116(1): 279 - 290. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. J. Maniotis, C. S. Chen, and D. E. Ingber Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure PNAS, February 4, 1997; 94(3): 849 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Christopher, A. Kowalczyk, and P. McKeown-Longo Localization of fibronectin matrix assembly sites on fibroblasts and endothelial cells J. Cell Sci., January 3, 1997; 110(5): 569 - 581. [Abstract] [PDF]
|
|
|
|
|
|
J. Apgar Increased degranulation and phospholipase A2, C, and D activity in RBL cells stimulated through FcepsilonR1 is due to spreading and not simply adhesion J. Cell Sci., January 3, 1997; 110(6): 771 - 780. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Pirttiniemi and T. Kantomaa Electrical Stimulation of Masseter Muscles Maintains Condylar Cartilage in Long-term Organ Culture Journal of Dental Research, June 1, 1996; 75(6): 1365 - 1371. [Abstract] [PDF]
|
|
|
|
|
|
J. Murphy-Ullrich, M. Pallero, N Boerth, J. Greenwood, T. Lincoln, and T. Cornwell Cyclic GMP-dependent protein kinase is required for thrombospondin and tenascin mediated focal adhesion disassembly J. Cell Sci., January 10, 1996; 109(10): 2499 - 2508. [Abstract] [PDF]
|
|
|
|
|
|
C Zhang, H Jenkins, M. Goldberg, T. Allen, and C. Hutchison Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract J. Cell Sci., January 9, 1996; 109(9): 2275 - 2286. [Abstract] [PDF]
|
|
|
|
|
|
M. Buschmann, E. Hunziker, Y. Kim, and A. Grodzinsky Altered aggrecan synthesis correlates with cell and nucleus structure in statically compressed cartilage J. Cell Sci., January 2, 1996; 109(2): 499 - 508. [Abstract] [PDF]
|
|
|
|
|
|
D. Mooney, R Langer, and D. Ingber Cytoskeletal filament assembly and the control of cell spreading and function by extracellular matrix J. Cell Sci., January 6, 1995; 108(6): 2311 - 2320. [Abstract] [PDF]
|
|
|
|
 |
|
 N Wang, J. Butler, and D. Ingber Mechanotransduction across the cell surface and through the cytoskeleton Science, May 21, 1993; 260(5111): 1124 - 1127. [Abstract] [PDF]
|
|
|
|
|
|
D. Ingber Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton J. Cell Sci., January 3, 1993; 104(3): 613 - 627. [PDF]
|
|
