|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 4 925-930, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
JM Carroll and LB Taichman
Graduate Program in Cellular and Developmental Biology, School of Dental Medicine, State University of New York, Stony Brook 11794-8702.
Involucrin, a component of the cornified cell envelope, is expressed specifically in differentiating keratinocytes of stratified squamous epithelia. To explore the regulation of involucrin expression, 3.7 kb of upstream sequences of the human involucrin gene was cloned into a plasmid containing a beta-galactosidase reporter gene and transfected into early passage keratinocytes and a variety of human cell types. The full-length construct gave maximal and tissue-specific expression. Deletion analysis showed that sequences between 900 and 2500 bp upstream of the transcriptional start site and the intron located between the transcriptional and translational start sites were required for maximal expression. Further analysis of the intron indicated that its effects on expression were independent of it being present in nascent RNA and suggested that sequences within the intron have regulatory activity. These results suggest that the involucrin intron operates in vivo to regulate expression in the epidermis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. L. Moulson, M.-H. Lin, J. M. White, E. P. Newberry, N. O. Davidson, and J. H. Miner Keratinocyte-specific Expression of Fatty Acid Transport Protein 4 Rescues the Wrinkle-free Phenotype in Slc27a4/Fatp4 Mutant Mice J. Biol. Chem., May 25, 2007; 282(21): 15912 - 15920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xie, S. Chang, Y. Oda, and D. D. Bikle Hairless Suppresses Vitamin D Receptor Transactivation in Human Keratinocytes Endocrinology, January 1, 2006; 147(1): 314 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Elias, N. Matsuyoshi, H. Wu, C. Lin, Z. H. Wang, B. E. Brown, and J. R. Stanley Desmoglein Isoform Distribution Affects Stratum Corneum Structure and Function J. Cell Biol., April 16, 2001; 153(2): 243 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie and D. D. Bikle Phospholipase C-gamma 1 Is Required for Calcium-induced Keratinocyte Differentiation J. Biol. Chem., July 16, 1999; 274(29): 20421 - 20424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Efimova, P. LaCelle, J. F. Welter, and R. L. Eckert Regulation of Human Involucrin Promoter Activity by a Protein Kinase C, Ras, MEKK1, MEK3, p38/RK, AP1 Signal Transduction Pathway J. Biol. Chem., September 18, 1998; 273(38): 24387 - 24395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie and D. D. Bikle Cloning of the Human Phospholipase C-gamma 1 Promoter and Identification of a DR6-type Vitamin D-responsive Element J. Biol. Chem., March 7, 1997; 272(10): 6573 - 6577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. LaPres and L. G. Hudson Identification of a Functional Determinant of Differentiation-dependent Expression in the Involucrin Gene J. Biol. Chem., September 20, 1996; 271(38): 23154 - 23160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lopez-Bayghen, A. Vega, A. Cadena, S. E. Granados, L. F. Jave, P. Gariglio, and L. M. Alvarez-Salas Transcriptional Analysis of the 5`-Noncoding Region of the Human Involucrin Gene J. Biol. Chem., January 5, 1996; 271(1): 512 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-L. Tu, W. Chang, and D. D. Bikle The Extracellular Calcium-sensing Receptor Is Required for Calcium-induced Differentiation in Human Keratinocytes J. Biol. Chem., October 26, 2001; 276(44): 41079 - 41085. [Abstract] [Full Text] [PDF]
|
|
