|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 103, Issue 4 999-1012, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
RE Stephens and G Prior
Marine Biological Laboratory, Woods Hole, MA 02543.
Serotonin, an activator of adenylate cyclase, stimulates motility in molluscan gill cilia and sperm flagella. To determine and compare potential targets of cAMP action, dynein was prepared from the lateral gill.cilia and sperm flagella of the mussel Mytilus edulis and the clam Spisula solidissima. In the flagella of both species, high-salt extraction removes about half of the ATPase activity, half of the alpha and beta heavy chains, and the outer arms. The dynein from both species sediments at 18-20 S, contains two or three intermediate chains, and three light chains. High-salt plus detergent removes most of the remaining dynein ATPase, alpha and beta heavy chains, and inner arms, also yielding a stable 18-20 S particle. In gill cilia of both species, high-salt extraction removes only 12-18% of the ATPase, up to 1/3 of the alpha heavy chains, an equivalent amount of beta heavy chain, and a subset of the outer arms. The dynein sediments at 18-20 S and, in Spisula, the heavy, intermediate, and light chains precisely co-sediment. High-salt plus detergent removes another 1/3 of the alpha heavy chains, an equivalent amount of beta heavy chain, and the remaining outer arms. The ATPase sediments mainly as a 13-14 S form showing considerable dissociation of co-sedimenting intermediate and light chains. The inner arms and at least half of the ciliary dynein ATPase activity remain unextractable, corresponding in mass mainly to an apparent beta heavy chain that is vanadate-cleavable. Cyclic AMP-dependent, calcium-independent phosphorylation takes place on specific dynein light chains in cilia but on only the dynein alpha heavy chain in flagella. Pre-activation of the flagella prevents subsequent addition of labeled phosphate. Phosphorylation has no effect on the steady-state ATPase properties. The single phosphate added to the flagellar alpha chain is located within the LUV1 vanadate photocleavage fragment. Considering the probable locus of the light chains and the site of the alpha heavy chain phosphorylation, both beyond the active site and toward the base of the molecule, these distinct phosphorylations may regulate dynein action by modulating arm flexibility or interaction.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Zilli, R. Schiavone, C. Storelli, and S. Vilella Molecular Mechanisms Determining Sperm Motility Initiation in Two Sparids (Sparus aurata and Lithognathus mormyrus) Biol Reprod, August 1, 2008; 79(2): 356 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Bakst and V. Akuffo Serotonin Localization in the Turkey Vaginal but not Sperm Storage Tubule Epithelia Poult. Sci., February 1, 2008; 87(2): 356 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Katow, S. Yaguchi, and K. Kyozuka Serotonin stimulates [Ca2+]i elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel J. Exp. Biol., February 1, 2007; 210(3): 403 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Jimenez-Trejo, M. Tapia-Rodriguez, D. B. C. Queiroz, P. Padilla, M. C. W. Avellar, P. R. Manzano, G. Manjarrez-Gutierrez, and G. Gutierrez-Ospina Serotonin Concentration, Synthesis, Cell Origin, and Targets in the Rat Caput Epididymis During Sexual Maturation and Variations Associated With Adult Mating Status: Morphological and Biochemical Studies J Androl, January 1, 2007; 28(1): 136 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Riffell, P. J. Krug, and R. K. Zimmer Fertilization in the sea: the chemical identity of an abalone sperm attractant J. Exp. Biol., May 15, 2002; 205(10): 1439 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Wyatt, J. R. Spurzem, K. May, and J. H. Sisson Regulation of ciliary beat frequency by both PKA and PKG in bovine airway epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L827 - L835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Inaba, S Morisawa, and M Morisawa Proteasomes regulate the motility of salmonid fish sperm through modulation of cAMP-dependent phosphorylation of an outer arm dynein light chain J. Cell Sci., January 4, 1998; 111(8): 1105 - 1115. [Abstract] [PDF]
|
|
|
|
 |
|
 K. F. Hoffmann and M. Strand Molecular Identification of a Schistosoma mansoni Tegumental Protein with Similarity to Cytoplasmic Dynein Light Chains J. Biol. Chem., October 18, 1996; 271(42): 26117 - 26123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. King, E. Barbarese, J. F. Dillman III, R. S. Patel-King, J. H. Carson, and K. K. Pfister Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000Light Chain J. Biol. Chem., August 9, 1996; 271(32): 19358 - 19366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Patel-King, S. E. Benashski, A. Harrison, and S. M. King Two Functional Thioredoxins Containing Redox-sensitive Vicinal Dithiols from the Chlamydomonas Outer Dynein Arm J. Biol. Chem., March 15, 1996; 271(11): 6283 - 6291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. King and R. Patel-King Identification of a Ca(2+)-binding light chain within Chlamydomonas outer arm dynein J. Cell Sci., January 12, 1995; 108(12): 3757 - 3764. [Abstract] [PDF]
|
|
|
|
|
|
M Salathe, M. Pratt, and A Wanner Protein kinase C-dependent phosphorylation of a ciliary membrane protein and inhibition of ciliary beating J. Cell Sci., January 12, 1993; 106(4): 1211 - 1220. [Abstract] [PDF]
|
|
|
|
|
|
C. Walczak and D. Nelson In vitro phosphorylation of ciliary dyneins by protein kinases from Paramecium J. Cell Sci., January 12, 1993; 106(4): 1369 - 1376. [Abstract] [PDF]
|
|
